You can also create reports or real-time PCR data markup language (RDML) files for quick import into qbase+ software.įig. If these values are out of range, you will need to troubleshoot your experiment.Įxport results for publication - Quickly export any charts or tables by right clicking in the window and selecting Save Image As (Figure 3) or Export to Excel (Figure 4). The efficiency should be within 90–110% and the R 2 should be >0.990. Check the efficiency and R 2 of the standard curve. Easily view up to six different charts or tables, such as the amplification plot, standard curve, gene expression chart, plate layout, or melt peak with the Custom Data View tab (Figure 2). Plate setup in the Plate Editor window.Īnalyze the data - a data file is automatically generated after a run with the gene expression module. This can be done before, during, or after a run.įig. Set up the plate - indicate position of unknown, no template controls (NTCs), and standard curve samples on plate (Figure 1).
#Qpcr data analysis excel template how to
An example of how to analyze a gene expression experiment in CFX Maestro software is shown below.
Similar to other real-time PCR software, CFX Maestro software offers several analysis modules, including quantification, melt curve, gene expression, allelic discrimination, and end-point analyses. With one software for all four systems, you can easily open and read files from any of the instruments, collaborate with other researchers, and move to another system without learning to use new software. All of Bio-Rad's real-time PCR detection systems include the powerful, easy-to-use CFX Maestro Software.
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